The evaluation of positive-staining chondrocytes was performed by using our previously published method [ 25].
As we show here, such training can be implemented as a flexible and concise module (~1-day home study, ~8-hour course time) on university courses by using our previously published training package designed for fly researchers (Roote and Prokop, 2013, G3 (Bethesda) 3: 353−358).
We have made the parsimonious tree by using our own data and data published elsewhere [ 11- 15, 15, 21, 22].
For sequencing analyses, all 12 coding exons and the non-coding exon 0 of GRN and exon 1 and exons 9 13 of MAPT were amplified by PCR using our previously published primers and protocols (Hutton et al., 1998; Baker et al., 2006).
> -wrap-foot> Exons 0 12 and the 3′-untranslated region of the GRN gene were amplified by polymerase chain reaction using our previously published primers and protocol (Baker et al., 2006; Gass et al., 2006).
Alternatively, for some samples the peptides generated by Lys-C were quantified using our previously published method of HPLC and MS coupled to capillary zone electrophoresis [ 26].
This PCR assay was developed in our laboratory by using primers published by Brakstad O.G et al., and Murakamy K., et al.[ 23, 24].
Our results contribute to hydro-ecological understanding by using the published literature to test for general cause-effect relationships between flow regime and terrestrial vegetation encroachment.
No analysable signals were obtained, even by using a published trick [10] with oxidation with a large excess of bromine.
The model adopted here was validated by using the published experiment and was in reasonable agreement with experimental data.
