Thus, by using optimized pre-UDPS protocols and effective data cleaning strategies, we have been able to increase the sensitivity for detection of genuine virus variants so that variants within the HIV-1 quasispecies that are as rare as 1 in 1000 can be reliably detected.
In fact, in a first set of experiments, we had initially failed to detect this band, and it is only by using very optimized protocols and very sensitive photographic films that we have managed to detect this band in brain extracts reliably.
The results we obtained suggest that, by using an optimized protocol, an efficient phage antibody library can be generated.
In summary, by using our optimized protocol in single cells from the lymphoblastoid cell line, we successfully detected a chromosomal deletion that was previously unidentified.
These results suggested that by using an optimized protocol and very high repertoire diversity, a compact and efficient phage antibody library can be generated.
Peptide was synthesized on a low loading Rink Amide MBHA resin at a 0.15 mM scale using a Focus XC automated solid phase peptide synthesizer (Aapptec, Louisville, KY) by using an optimized protocol reported previously.
AAV particles were produced by co-transfection of the helper plasmid pDG [ 38] and the transgene-encoding AAV vector into HEK293-T using optimized calcium phosphate transfection protocols.
Using optimized amounts of DNA, the TDP3 protocol demonstrated one or other of seventeen different HPV types in 47 of 65 (72%) abnormal cytology samples HPV negative by the regular GP5+/GP6+ protocol.
Platelets from whole blood were isolated using an optimized protocol using centrifugal separation in an acid-citrate-dextrose (ACD) buffer, which uses citrate as an anticoagulant.
To measure the accuracy of our UDPS protocol, the SG3Δenv plasmid was diluted to 1 copy, amplified using the optimized protocols described above and subjected to UDPS.
