A negative control to each section was prepared by using normal rabbit serum instead of the primary antibody.
Nonspecific binding of primary antibody was blocked by using normal swine serum (NSS, in Tris-buffered saline (TBS) (1 5), 100 μl/slide) for 10 minutes of incubation.
Immunostaining was performed as follows: the sections were washed in BSA buffer (1% BSA and 0.15% glycine in PBS) followed by blocking for 30 min using normal goat serum (1 20 dilution in BSA buffer).
Sections were then pretreated by microwaving in citrate buffer and blocked using normal goat serum (1 10 for 45 min).
Nonspecific binding was blocked by using 10% normal rabbit serum and 0.5%.
Nonspecific binding was blocked by using 10% normal goat serum and 0.5% casein in PBS with for 30 min.
The sensitivity, specificity, and positive and negative predictive values of lactate dehydrogenase (LDH) for the diagnosis of PCP were calculated by using the normal high serum LDH level for our laboratory of 210 units/mL as a cutoff point.
by Using a Serum, Says Dr. Vincent of France.
The fixed cells were permeabilized with 0.3% Triton X-100, and nonspecific binding was blocked by using 1% normal goat serum.
S. pneumoniae was detected by using pneumococcal anti-serum.
