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Altogether, these analyses demonstrate that much of the inter-tumoral GBM heterogeneity can be modeled by using neural stem cell culture conditions.
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The GBM cell lines GBM L1, GBM L2, GBM4, GBM6, CSC020, CSC014, BAH-1 and HK301 were established in-house directly from patient tumors using neural stem cell conditions, as recently described by us [ 25].
Neural transplantation therapy using neural stem cells has received as potential treatments for neurodegenerative diseases.
Stem cell therapy using neural stem cells (NSCs), adult stem cells such as mesenchymal stem cells (MSCs), etc. has been investigated as a promising tool for the treatment of SCI [9].
GBM-targeted therapy using neural stem cells and MSCs as vehicles for therapeutic agents is a promising strategy.
ANN models were constructed by using neural-network software (Statistic Neural Networks, version 4.0).
The formation of neurospheres in vitro in U87MG and U343MG cell cultures was induced by using specific neural stem cell (NSC) medium (Supplementary Figure 1A).
In vitro tests using mouse neural stem cells seeded on nanopillars showed that the cells sense the nano-sized topography and respond accordingly by orienting themselves.
We used fetal neural stem cells as a model for the normal cell type of origin of the brain tumor stem cells.
Neuronal differentiation was then induced by subculture of neural stem cells using 0.05% Trypsin/EDTA in P-ACM for 14 days.
Other companies, looking to sidestep both Geron's patents and the ethical issues surrounding embryonic cells, are using so-called neural stem cells on which they have obtained patents.
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