Sentence examples for by using nested reverse from inspiring English sources

RNA was detected by using nested reverse transcription PCR (RT-PCR) specific for viral nucleoprotein gene (5 ).

The 11 specimens were then examined for sapovirus by using nested reverse transcription PCR (RT-PCR) as described (3 ).

CoV was detected by using nested reverse transcription PCR (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene (12 ) (see Table 1 for assay oligonucleotides).

To understand the possible association between salivirus/klassevirus and diarrhea, we conducted prevalence studies by using nested reverse transcription PCR (RT-PCR).

CoV RNA was detected by using nested reverse transcription PCR with the degenerated primers to amplify the RNA-dependent RNA polymerase (RdRp) gene (6 ).

We tested for KFD virus by using nested reverse transcription PCR (RT-PCR) and Taqman-based RT-PCR at the National Institute of Virology (Pune, India) (7 ) and/or intracerebral injection of the serum into suckling mice at the Virus Diagnostic Laboratory, Shimoga (Technical Appendix).

By using Nested, both are guaranteed.

‡p value calculated by using nested analysis of variance.

HEV RNA was amplified by using a nested reverse transcription PCR for the ORF2 gene as described (27 ).

HEV RNA was detected in the serum sample by using a nested reverse transcription PCR for open reading frame (ORF) 1 or ORF2 genes (11, 12 ).

The molecular detection of the virus was performed by using a nested reverse transcription PCR (RT-PCR) described by Sall et al. (12 ) or a real-time RT-PCR described by Weidmann et al. (13 ).