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Red arrows indicate the genome position of genes identified by using natural variation.
However, when we examined the genomic location of QTL clusters and genes identified by using natural variation, we found that the QTL clusters often contained multiple genes identified by using natural variation (Figure 3).
Genetic analyses of root system architecture have been performed in a variety of rice lines; however, no genes controlling soil-surface rooting have been isolated by using natural variation.
Because most of the genes functionally characterized by using natural variation were identified as genes responsible for QTLs, it was not unexpected to find that most of their genomic locations were included in QTL regions associated with those same traits (Figure 2).
For example, on the long arm of chromosome 1, which contains the largest QTL cluster region, there were four genes that had been identified by using natural variation: Pi37 for blast resistance (Lin et al. 2007), qSH1 for seed shattering (Konishi et al. 2006), qNPQ1-2 for photosynthetic capacity (Kasajima et al. 2011), and sd1 for culm length (Sasaki et al. 2002).
Thus, by using natural variation to search for mutant suppressors and genetic interaction, we have detected the gene, sol, for leaf morphology.
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This information will set goals for biofortifying phytonutrients in crops, which can be achieved by metabolic engineering, either using natural variation or genetic engineering.
Our results reveal new aspects about effector recognition by Pto and demonstrate the value of using natural variation to understand the interaction between resistance proteins and pathogen effectors.
Both of these can be functionally evaluated in tomato using natural variation for drought adaptive traits.
The treatment of fluoride rich waters by using natural materials shows that there is a significant variation in FTIR spectrum before and after treatment.
Genome-wide association studies (GWAS) can partially overcome the problems associated with QTL studies by using the natural variation present in a large number of geographically and genetically distinct individuals from many populations.
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by using natural variants
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by studying natural variation
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by using natural gas
by evaluating natural variation
by using natural spline
by using natural dispersal
by using natural opportunity
by using natural biocontrol
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by using natural evolution
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