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(5) and (6), best fit estimations for k on and k off can be calculated by using multiple radioligand concentrations.
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An extensive investigation using multiple radioligands and modulators identified the presence of at least four pharmacologically distinct binding sites on P-gp [ 35].
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20 In addition, it is shown that by using a radioligand for DAT nigrostriatal damage can be detected years before the onset of motor signs of PD.
The role of neuroinflammation in brain tumor-associated depression depression could also be further studied by molecular imaging approaches targeting activated microglia, e.g., by using PET radioligands for the translocator protein [65].
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These mechanisms might be revealed by using particular receptor radioligands in a positron emission tomography (PET) experiment [31], [32].
Starting with the proof of existence by purification of the lac repressor protein by Wally Gilbert, using radioligand binding measured by equilibrium dialysis as an assay (39), radioligand binding quickly became a successful method to identify receptors for hormones and neurotransmitters.
The ultrahigh specific activity of the radioligand was consistent with our previous results by using 11 C]CH4 as a starting precursor [27].
The IC50 values (the concentration of competitor required to inhibit 50% of the radioligand binding) of the peptides were calculated by using Origin 8.0 (MicroCal).
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CEO of Professional Science Editing for Scientists @ prosciediting.com