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It took us 60 minutes 31 seconds to finish the complete alignment for these 21 sequences by using multiple alignment method [14] whereas we just need 6 seconds to get the result.
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Pair-wise identity in the ORF1b and full-length capsid protein of strain Nausika/08/ITA to a selection of AstV strains was determined by using multiple alignments generated with Bioedit software package version 2.1 (2.1).
Given the high accuracy reached by secondary structure predictors using multiple alignments (e.g. Frishman and Argos, 1997; Jones, 1999; Pollastri and McLysaght, 2005; Rost and Sander, 1995; Thompson and Goldstein, 1997), the present study offers the possibility of acquiring a good quality information to predict tertiary structures from sequence data only, using a minimal number of parameters.
In ambiguous cases, putative regulatory elements were validated by phylogenetic footprinting [ 53] using multiple alignments for upstream non-coding regions of orthologous genes.
The isolate sequences were genotyped into assemblage using multiple alignments implemented by ClustalW [ 28] with previously defined reference sequences retrieved from GenBank database.
Finally, the classified families were verified by using multiple sequence alignment of LTRs and IRs.
Unique identifiers between template sequence and target sequence were compared to determine homology within the sequence by using multiple sequence alignment available in Command Line Calculator (CLC) drug discovery workbench.
Comparison of the 18S rRNA gene sequences of these isolates with 22 other published reference sequences, derived from a variety of human and animal Cryptosporidium isolates by using multiple sequence alignment and phylogenetic analysis, showed that the 11 isolates fell into four main groups.
Both sensitivity and specificity can be dramatically improved by using multiple sequence alignments as queries.
ERPIN (Easy RNA Profile IdentificatioN) is an algorithm used to define RNA motifs by using multiple sequence alignments and secondary structure consensus.
We identified conserved regions by using multiple sequence alignments of AstV-MLB1 amino acid sequences to all fully sequenced astrovirus genomes.
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