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This goal is achieved by using multiple PEs with a CU.
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By using multiple narrators, McBurney allows for every possibility.
In this paper, we propose a distribution implementation for the particle PHD filter using multiple processing elements (PEs) and a central unit (CU).
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Thus, estimates of Ppc made by using Ppl (Pes) measurements may overestimate surrounding pressure as Ppl is increasing.
After low-density cells were collected and washed in PBS-FCS, F4/80+ cells were isolated by using F4/80-PE and anti-PE coated magnetic microbeads (MACs, Miltenyi Biotec).
Biotinylated antibodies were labeled by using Streptavidin-PE-CY5 (Invitrogen, SA1012, Carlsband, California, USA).
One or two days after transfection, cell-surface expression of CR3 was measured by using a PE-conjugated anti-CD11b (clone ICRF44) antibody and PE-conjugated isotype control.
Cluster generation was done by using TruSeq PE Cluster Kit version 2.5 (Illumina) on a c-bot.
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Fas expression was additionally examined by using PE-conjugated hamster anti-mouse Fas antibody (clone Jo2) or PE-conjugated hamster IgG2 (BD Biosciences) as a control antibody.
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CEO of Professional Science Editing for Scientists @ prosciediting.com