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A total of 187 colonies were typed by using multilocus variable-number tandem-repeat (VNTR) analysis as described (9 – 12 ).
This study aimed to determine the S. Enteritidis lineages in Japan over the last few decades by using multilocus variable-number tandem repeat analysis (MLVA).
Tissues with cycle threshold (Ct) values <34 (1/case) were typed by using multilocus variable-number tandem-repeat analyses (MLVA) for 11 loci, as described (2, 5 ); results were compared with known MLVA typing data from the Netherlands.
We characterized the isolates by using multilocus variable-number tandem repeat analysis (MLVA) and multiple antigen sequence typing (MAST) to partially sequence the genes encoding pertactin (prn), B. pertussis toxin S1 subunit (ptxA, also designated ptxS1), B. pertussis toxin promoter (ptxP), and tracheal colonization factor A (tcfA).
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To determine detailed associations between environmental and human clinical samples, we examined the genetic diversity among these samples by using multilocus variable number of tandem repeats analysis (MLVA) (13 ).
As there is limited information on the genetic background of S. sonnei in Malaysia, this study aimed to characterize Malaysian S. sonnei and to evaluate the prospect of using multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) for subtyping of local S. sonnei.
Reduced genetic diversity possibly resulted from introduction of pertussis vaccines We used multilocus variable-number tandem repeat analysis and multiple antigen sequence typing to characterize isolates of Bordetella pertussis strains circulating in Denmark during periods with and without pertussis vaccination coverage.
Samples with sufficient DNA load (cycle threshold [Ct] value <32) were typed by using 2 multilocus variable-number tandem-repeat analyses (MLVA) genotyping methods (MLVA-12 and MLVA-6), and the multispacer sequence typing method (3 – 5 ).
Isolates were subtyped by using multilocus sequence typing (MLST).
By using multilocus sequence typing (MLST) (9 ), the outbreak strain was classified as ST45-MRSA-IV.
Screening for sequence type (ST) 131 was conducted by using PCR and confirmed by using multilocus sequence typing (7, 8 ).
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