The purities of proteins were close to 95% as determined by SDS-PAGE, with the exception of SjGP-4, in which a degradation product was observed and confirmed by Western blotting using mouse immune sera against Sj26GST or Pym-F4, retained in our lab (data not shown).
The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa (GFP) by using mouse peritoneal macrophage and cytoxan (CTX) induced immunosuppression models.
Experiments to distinguish between parasite and host factors are possible using mice without immune systems or by blocking the actions of specific factors.
In order to overcome the immunosuppression caused by maternal antibody, several immune strategies were explored in this paper using mouse model and influenza vaccines.
Using mouse primary microglia and RAW264.7 cells, we found that some thiourea derivatives significantly enhanced microglial Aβ phagocytosis and suppressed microglial immune responses, as evidenced by the reduced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2).
In follow-up studies using mice with human-like immune systems, the researchers showed that the tissue transplants were not rejected during a three month trial.
But Allison started looking at how the immune system fights disease, using mice as study models, and capitalized on a critical discovery: T-cells require two signals to attack a target effectively.
By using immune-competent mice in our study, we better modeled the effects of radiation on the immune system, clearance of radiated cancer cells by the immune system, and possible immunogenicity of radiated tumours (abscopal effect).
By using a mouse model and immune-competent cell systems, we studied the role of D2 in controlling the innate immune response.
In the late 1960s, using mice, the researchers injected viruses and retroviruses and again traced the resulting immune complexes to the kidney.
