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High speed and precise flow control can be achieved by using microvalves on a chip.
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Fluidics were added to enable the extracellular media to be exchanged with various solutions (media or stimulation solutions) and a collection port/channel to enable off-line sample characterization, in this case via MS. In order to enable temporal control of the media surrounding the cells, we used microvalve-controlled stimulation channels.
For example, by using the combination of the gel mixed solution and microelectrodes on the substrate of the microchannel, we can expect high-speed response of gel microvalves within several tens milliseconds [19],[24],[25].
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Using on-chip microvalves, the chamber can be connected to the sample source during the "loading" step, and to the CE separation channel during the "injection" step.
To further demonstrate the material versatility, we performed three critical microfluidic applications on epoxy resin-based microdevices, including multilayer pneumatic microvalves, on-chip PCR with improved efficiencies, and ultra-high-speed flow cytometry imaging of microbeads at a flow rate of 8.3 m/s.
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