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Target prediction was performed by using miRU algorithm (Dai & Zhao 2011; Zhang 2005) with default parameters.
All the introns with 30-nt flanking sequences at both ends were subjected to miRNA (miRBase (Griffiths-Jones et al. 2008), release 18) binding site prediction by using miRU algorithm (Dai & Zhao 2011; Zhang 2005).
Target prediction was performed by using miRU algorithm [ 17, 18] with default parameters.
To gain deeper functional insights of the miRNAs regulated by the target mimics, large-scale target prediction was performed by using miRU algorithm [ 21, 22].
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However, the discriminatory power observed in this large strain sample by using MIRU-VNTR and spoligotyping is already so high that a significant increase in the number of genotypes as defined by IS6110 RFLP would not be expected.
Many countries now routinely type M. tuberculosis isolates by using MIRU-VNTR typing.
Drug-susceptibility testing and genotyping by using MIRU-VNTR and spoligotyping were performed by the Supranational Reference Laboratory at the University of Massachusetts Medical School.
The latest addition to the fingerprinting methods for M. tuberculosis, MIRU-VNTR [ 16, 17], is efficient and is being adopted by several labs and we are currently analyzing our isolates using MIRU fingerprinting.
Thus, further characterization of these two isolates was undertaken using MIRU-VNTR.
Genotypic profiling was performed on these isolates using MIRU-VNTR.
Demographic information (sex and age), spoligotyping and MIRU-VNTR patterns were compared to the international SpolDB4.0 database using MIRU-VNTR plus, freely available web-based software [ 18].
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