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Nucleus pulposus cells were isolated by using methods reported by Hiyama et al., 2007 [ 1].

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Extra- and intracellular lactate levels were quantified using methods reported by us.

If explicit survival data were not provided, they were calculated from the available numerical data using methods reported by Parmer et al. [ 13].

When these statistical variables were not directly provided in the original articles, they were calculated from available numerical data using methods reported by Parmar et al. [ 16].

We estimated residential exposures to 2006 annual mean concentrations of NO2 using a national land use regression model (LUR) developed from National Air Pollution Surveillance (NAPS; http://www.ec.gc.ca/rnspa-naps/) monitoring data using methods reported by Hystad et al. (2011).

Primary ovarian cancer cells were obtained from the ascites of patients undergoing surgery for ovarian cancer and cultured in RPMI 1640 with 10% fetal bovine serum (Invitrogen, CA USA), using methods reported by Szlosarek [ 12].

Nucleus pulposus cells and annulus fibrosus cells were isolated from the lumbar discs of 11-week-old Sprague Dawley rats (n = 32) using methods reported by Hiyama et al. [ 3].

The aliquots were shipped frozen to the laboratory; p,p′ -DDE, o,p′ -DDT, and p,p′ -DDT were assayed using methods reported previously by Gammon et al. (2002).

The constructs were introduced into G. lozoyensis by Agrobacterium-mediated transformation using method reported by Zhang et al. [ 21] with slight modification.

To verify function of the predicted pneumocandin gene cluster, glnrps4 and glpks4 deletion were conducted using method reported by Zhang et al. [ 21].

DNA was extracted, amplified, cloned, and sequenced by using methods formerly reported [ 52- 54].

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