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This study demonstrated the possibility to regulate fatty acid composition by using metabolic engineering approaches.
Controlling the availability of the energy equivalent ATP at a sufficient level during the production phase, forms an interesting lead to optimize the biocatalyst by using metabolic engineering.
In this paper, by using metabolic engineering techniques, the more efficient biosynthetic pathway of α-pinene was successfully assembled in E. coli BL21 DE3) with the heterologous hybrid MVA pathway, GPPS2 and α-pinene synthase (Pt30).
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By using plant metabolic engineering approach, tremendous changes at metabolic level in different plants have been done.
In this study, we used metabolic oligosaccharide engineering methods to identify the sialoglycoproteins modified by azido-sugar in metabolically engineered pancreatic cancer cells when the cells were over-supplied with sialic acid substrate analog.
Manipulation of metabolic pathways by using genetic engineering in microalgae is relatively easy due to its unicellular formation.
In this study, we performed a side-by-side comparison of three E. coli strains frequently used for metabolic engineering projects.
2D [13COSY] COSY NMR is used by the metabolic engineering community for determining 13C 13C connectivities in intracellular compounds that contain information regarding the steady-state fluxes in cellular metabolism.
Most biosynthesis pathways used in metabolic engineering applications have been discovered manually, requiring a tedious search of existing literature and metabolic databases.
EFMs are also used in metabolic engineering to improve product yield [ 3],[ 4].
Here, we reviewed several computational applications for metabolic engineering by using genome-scale metabolic models of microorganisms.
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