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It is performed by using maximum dilution of antibody which can confirm 90% reduction in viral infectivity after virus neutralization [ 10].
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Primer efficiency was tested for each primer pair (Additional file 1: Table S1) over five dilution points using Maxima SYBR Green qPCR master mix (Fermentas).
RT-qPCR was performed using Maxima SYBR Green qPCR mastermix (Fermentas) in 10 μl reactions containing 2 μl of 1 15 cDNA dilution.
qRT PCR was performed using Maxima SYBR Green qPCR mastermix (Fermentas) in 10 μl reaction mixtures containing 2 μl of 1 15 cDNA dilution.
By using this dilution, the CFE samples typically yield Sav FBS = 20 30 μM.
SiHa clones A and B were isolated from the wild-type cell population by using limiting dilution.
The MIC values for these polymers were determined by using the broth dilution method.
Body composition was measured by using the deuterium dilution technique.
bELISA titers were determined by using fourfold serum dilutions; others were determined by using twofold dilutions.
Positive samples were quantified by using serial dilutions.
One can "titrate" the bovine serum (determine the level of specific anti-trypanosome immunoglobulins) by using serial dilutions of the serum and determining the end point, the highest dilution still giving a positive test result.
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