Here, we report an approach that increases experimental throughput by using a master template to generate a single standard curve for the estimation of mRNA copy numbers from all genes.
The present study was meant to investigate the rheological behavior of various PMAs before and after aging (Rolling Thin Film Oven Test (RTFOT) and Pressure Aging Vessel (PAV)) by using rheological master curves in combination with Gel Permeation Chromatography (GPC).
Primers specific for β-actin, IL-6, G-CSF, NOS2, IL-1β (Invitrogen) were used and relative gene expression determined by using RT Real-Time SYBR Green/ROX PCR master mix (Takara, Japan) on an LightCycler 480 quantitative PCR machine (Roche).
All quantitative real-time polymerase chain reaction (qRT-PCR) samples were performed by using SYBR Green PCR master mix (CWBIO, China).
Quantitative real-time PCR was performed by using SYBR Green PCR master mix (Roche, USA) on an ABI 7500 real-time PCR machine.
QPCR was performed by using SYBR Green PCR master mix and 7900HT Thermal Cycler (Applied Biosystems) at typical amplification parameters (50°C for 2 min and 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min).
To link particular nurses to activities performed in SARS patients' rooms, we identified nurses' signatures on patient charts by using a master list of signatures provided by the CCUs.
To investigate the potential proteomic alterations induced by low intensity endurance exercise in mdx mouse muscles, proteome profile of quadriceps isolated from WT-Sed and MDX-Sed or MDX-Ex mice were compared by using Image Master 2D Platinum 6.0.
PCRs to amplify a fragment of 17-kDa protein antigen were carried out in a total volume of 25 μL by using Taq Master Mix (QIAGEN) in a Gradient Mastercycler (Eppendorf, Westbury, NY, USA).
