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To overcome the time consumption of Smith Waterman algorithm, Lipman and Pearson proposed FASTA tool in 1985, which takes a given nucleotide or amino acid sequence and searches a corresponding sequence database by using local sequence alignment.
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However, some TB classifiers are currently used by biologist users in their workflow: GoPubMed uses local sequence alignment of words and GO terms for powering its ontology-based search engine (17), or Textpresso uses regular expressions for recognizing GO terms and assisting WormBase and TAIR biocurators (18).
We annotated the 454 sequences by using local BLASTx to align the consensus sequences from the assembled contigs and the singleton sequences to the Uniref50 15.4 and the TAIR9 Arabidopsis thaliana annotated protein databases using an E-value threshold of 10−11.
By using local blast alignment, 1,674 of the metagenomic sequences were mapped to 300 antibiotic related genes (Additional file 6).
Batch blast of the unique sequences was carried out by using local Blast software (ver.2.2.25+) available from ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/.nih.gov/blast/executables/blast+/LATEST/
The analysis of sequence redundancy was performed by sequence comparison using local BLASTN through a clustering system running under an alpha version of Biopipeline® and by using the Cap3 contig assembly program [ 22].
Comparison of whole chromosome sequences was performed by MAUVE software using local collinear blocs (LCB).
In contrast, conventional techniques deliver solutions in sequence using local path continuation.
To improve imputation accuracy of rare variants, various approaches have been suggested, including increasing the sample size of the reference panel, using sequencing data from study-specific samples (i.e., specific populations), and using local reference panels by genotyping or sequencing a subset of study samples.
To avoid alignment efficiency biases caused by the presence of the insertion, the allele ratios were subsequently requantified using local realignment with the reference and mutated sequences.
Such markers are typically identified either by global multiple sequence alignment algorithms, or simply using reciprocal best matches using local alignment tools like BLAST.
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