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Environmental samples and patients' stool samples were collected and were tested at different institutions by using local protocols, primarily reverse transcription PCR (12 ).
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Finally, two different VOT protocols have been used, as StO2 monitoring was performed using the local protocol of each center.
The serum samples from healthy adult blood donors aged 18 65 years were obtained from the University Hospital of Ferrara Blood Center, and samples from children and from adults 66 85 years of age were obtained from the Clinical Analysis Laboratory, University Hospital of Ferrara, by using a protocol approved by the local county ethics committee at University of Ferrara.
The communication between the utility company and the consumers' smart meters is done through the local area network (LAN) by using appropriate communication protocols.
In localized routing protocols, by assuming each node has position information, the routing decision is made at each node by using only local neighborhood information.
This was dictated by the local protocols.
Genomewide array analysis was performed before ANKRD11 analysis in all patients using different array platforms according to local protocols by the referring institutions.
The local citrate anticoagulation protocol was validated by using Lynda equipment.
We found that local protocols for pain management, when available, mostly recommended the use of paracetamol and NSAIDS, while opioids were mentioned by six EDs only.
Embryo quality was evaluated for all available embryos on day 3 of culture by the local embryologist using a protocol-defined guideline based on the following parameters: number of blastomeres, degree of fragmentation, blastomere size uniformity and presence or absence of multinucleation.
FISH was performed according to local protocols.
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