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Using a 3-D printer, researchers "print" organs by using live cells as "ink" and layering them in a precise pattern, leaving them space to grow into fully formed organs.
By using live cells we can negate a host of delivery limitations leading to better therapies.
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Here by using live cell confocal microscopy and in vitro reconstitution, we report that NDRG1 is involved in recycling the adhesion molecule E-cadherin thereby stabilizing it.
By using live cell imaging, we found that the fusion of autophagosomes to lysosomes was significantly impaired in SigR1-deficient cells as indicated by the minimal loss of GFP fluorescence and maximal colocalization of RFP and GFP signals.
Thus far, a group of cancer specific aptamers were generated by using live cell-SELEX, some of them have been successfully used for cancer detection and targeting therapy [ 20– 31].We have developed a gastric cancer specific DNA aptamer cy-apt 20 by employing live cell-SELEX.
By using live cell imaging we show where the two encoded proteins are expressed during sexual stage differentiation, especially during zygote development when apical polarity is established and the apical complex is formed, resulting in the fully differentiated and invasive ookinete.
Many are complex proteins that can be synthesised only by using living cells.
We then confirmed this finding for HeLa cells exposed to StxB by using live-cell spinning disk confocal imaging.
By using live-cell imaging, we investigated CEN3-MT interaction in further detail.
When directly monitoring the segregation of chromosome 2 in bub3Δ cells by using live-cell imaging, we observed only a slight increase in mis-segregation compared to wild-type cells (Fig 2B; Vanoosthuyse et al, 2004).
However, by using live-cell imaging of polarized, motile Swiss 3T3 fibroblasts, Rac activity was observed in a juxtanuclear region that was consistent with the ER or the Golgi [ 51].
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