Free Feet works by using laser assistive technology to act as a visual cueing system.
Today, researchers can induce the photoacoustic effect by using lasers.
Raman spectra of SWNTs on either silicon wafers or quartz plates were collected from JY-T64000 Raman spectroscopy under ambient conditions by using excitation laser lines of 514.5 nm (2.41 eV) or 632.8 nm (1.96 eV) from an air-cooled Ar+ laser.
Confocal laser scanning microscopy (CLSM) observations were performed with a Leica TCS SP2 AOBS apparatus (Leica, Wetzlar, Germany), as described [ 20], by using excitation spectral laser lines at 405, 488, 594, and 633 nm.
FRET was carried out using two laser lines; firstly the 488 nm line of an Argon laser (Lasos) set at 6.1A using 5% laser power and a pinhole of 1. Secondly a 543 nm line from a HeNe laser (Lasos) using 100% laser power with a pinhole of 1.
Furthermore, by using a laser irradiated line instead of a spot as the seed for these architectures, overall reproducibility was significantly improved.
Ablation of contractile stress fibers was achieved by using full laser power of 405 laser line (3I Marianas microscope) for 3 x 200 ms pulses.
The fluorescence of GFP and FM4-64 was imaged simultaneously by using the exciting laser line 488 nm, but separate emission detectors (490 – 543 nm and 667 – 746 nm, respectively).
Localization on the membrane and interaction of PC-PLC with HER2, EGFR, and HER3 were investigated on HER2-overexpressing and HER2-low breast cancer cell lines, by using confocal laser scanning microscopy, flow cytometry, cell-surface biotinylation, isolation of lipid rafts, and immunoprecipitation experiments.
