Sentence examples for by using laser capture from inspiring English sources

Therefore, we sought to further support the above results by using laser capture microdissection and array analysis to quantify mRNA levels selectively in beta cell-enriched samples [ 26].

Furthermore, a loss of allelic heterozygosity in a specific cancer-related chromosomal region was demonstrated in some HT harbouring galectin-3-positive follicular cells, by using laser capture microdissection.

A study by Hinshelwood and colleagues [ 103] (2009) assessed hypermethylation of the p16ink4a promoter region of vHMECs in the first few population doublings following selection by using laser capture and found that p16ink4a was silenced prior to hypermethylation.

To further support this diagnosis of OvHV-2 infection, we attempted to detect viral DNA in the vascular wall of the foal's hepatic arterioles that contained fibrinoid necrotizing lympho-histiocytic vasculitis by using laser capture microdissection on sections stained with hematoxylin and eosin (10 ).

Specific allelic loss for microsatellite D7S-2468 was demonstrated in three out of three focal undefined lesions (FULs) associated to HTs and in four out of six foci of micro-PTCs discovered in such diseases, by using laser capture microdissection followed by DNA extraction and PCR.

Then, the epithelia of KCOT, RC, and OM samples were carefully dissected by using laser-capture microdissection (LCM) as our previous description [ 9].

A transcriptome from a single tissue can now be obtained by using laser-capture microdissection, an approach whereby a cell is physically isolated from the surrounding tissue [ 25].

The expression of Ang1 in clinical breast cancer specimens was analysed by using laser-capture microdissection and reverse transcriptase-linked polymerase chain reaction (RT-PCR) on RNA isolated from the samples.

† T7 IVT systems may be used by changing the promoter sequence of the modified primer to a T7 binding sequence * Modified Eberwine Amplification ** As described by Xiang In clinical and research settings using laser capture microdissection or other strategies where very small amounts of starting RNA (10 100 ng) are available, multiple rounds of amplification may still be necessary.

Disc tissue was studied using laser capture microdissection to harvest cells, followed by microarray analysis as previously described [ 19].

This was accurately achieved using laser capture microdissection, as described in a recent study [12].