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The problem was only solved by using large volumes of very dilute solutions of both receptor and substrate.[ 15] The Ka values obtained for 6– 11 binding Et4N+Cl− in water-saturated chloroform are summarized in Table 1.
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The LODs in this study are generally lower than values reported in the literature, which can be explained by using large-volume injection.
By using large-volume injection (LVI), a major part of the sample can be injected, thereby lowering the detection limits in comparison with traditional GC injection techniques where only a small part of the sample (~1 μl) is introduced into the GC.
This may be because the CSF smears were performed, using large volumes of CSF (5 10 mls) by a dedicated technician in a research laboratory.
Early chip protocols often required more than 25 µg of total RNA, which could only be obtained by using large blood volumes.
The cough can also be increased with prior lung insufflation; it is possible to generate higher expiratory flows by using larger lung volumes.
A higher sensitivity could probably be achieved by using larger CSF extraction volumes (5-20 ml), but in general this is not practically possible.
However, cubic presses cannot be easily scaled up to larger volumes: the pressurized volume can be increased by using larger anvils, but this also increases the amount of force needed on the anvils to achieve the same pressure.
Conclusion: Hemostasis in cases of post-partum bleeding caused by low-lying placenta/placenta previa can be achieved by using a large volume, fluid-filled tamponade balloon.
Unfortunately not every control is inherently logical, and much has to be done via a two-stage process; for instance, to change the cabin temperature you must first hit the climate button haloing the LED display, then input what you need on said display by using a large, volume-like knob.
However, future studies using large colonial species should consider the ratio of colony volume to medium volume in the chamber to avoid possible artifacts associated with resource exhaustion by the largest colonies.
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