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By using increasing concentrations of TN122-eGFP, the efficiency of protein transduction measured by flow cytometry could be enhanced).
We have studied the effect of target length on the signaling of our E-DNA sensor by using increasing concentrations of complementary targets of different length.
In chick embryos, treatment by using increasing concentrations of RA antagonist, BMS453, showed that successively more posterior rhombomere boundaries required progressively higher concentration of endogenous retinoic acid for their correct positioning [ 125].
To this end, we first examined the effect of VFL on cell viability of HT1376, 5637, SW780, T24 and UMUC3 bladder epithelial tumour cells by using increasing concentrations of VFL (0 100 μM) treatment for 48 h.
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PTB was partially silenced in HeLa cells by using increasing concentration of siPTB.
Several 17-DMAG-resistant sublines, derived from parental H3122 and H2228 cell lines, were established by stepwise selection using increasing concentrations of 17-DMAG, as described in the Materials and Methods section.
All measurements were conducted in 3 ml cell culture medium and started with a short baseline, followed by a two-step uptake study using increasing concentrations of 125I-EGF (Table 1), followed by a retention measurement over night in cell culture medium.
TGF-β1 activity was quantified by comparison with a standard curve using increasing concentrations of human TGF-β1.
[GSH] was calculated by interpolation on a curve constructed using increasing concentrations of GSSG (0.1 10 nmol).
TGF- β1 levels were quantified by comparison with a standard curve using increasing concentrations of human TGF- β1.
Using increasing concentrations of ethanol followed by propylene oxide, the cells were embedded in epon resin.
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