Sentence examples for by using immunoblot analysis from inspiring English sources
Overexpression of DbpA and DbpB was verified by using immunoblot analysis probed with a mixture of a FlaB mAb and anti-DbpA or -DbpB sera as described in an earlier study [15].
To investigate the levels of activation of STAT3 in the treatment-responsive GCB lymphoma relative to ABC lymphoma, we selected eight patient tumor derived cell lines for study and examined levels of total and activated STAT3 by using immunoblot analysis.
We immunoprecipitated Bcl-2 using anti-FLAG mAb-conjugated agarose (M2-agarose; Sigma) and probed for Bim, Bmf, Bax, Bak, Puma and Bad after treatment with paclitaxel for 12 h by using immunoblot analysis.
To gain insights into the molecular mechanism of the antitumor efficacy of VEGF inhibition, we examined the expression of molecules acting downstream of VEGF signaling, after Bev and Pazo treatment, by using immunoblot analysis.
To mechanistically understand the photoactivated hypericin-induced apoptosis of RINm5F insulinoma cells, we investigated if photoactivated hypericin impinged on the pro-apoptotic mediators cleaved caspases-3, cleaved caspases-9 and the apoptotic index Bax-to-Bcl-2 ratio by using immunoblot analysis.
Although we were able to confirm modulation of MEF2A (pThr312) by XMD8-92 usimmunoblotblot analysis, direct confirmation of the additional biomarkers was not possible due to a lack of high quality antibodies.
The total amount of CD81 receptor obtained by the single-step affinity purification was determined by using quantitative immunoblot analysis.
Next, to examine the mechanism by which BRACs inhibited the interactions of MMP2 and MMP9 with HER2, ERK, and JNK, we pretreated MDA-MB-453 cells with or without BRACs for 24 h and then performed an immunoprecipitated assay with MMP2 and MMP9 antibodies followed by analysis using immunoblotting.
Activities of the conventional MAPKs were monitored by their phosphorylation levels using immunoblotting and immunocytochemistry analysis.
More recently, however, evidence suggestive of TPMV infection was found in a Laotian immigrant with a febrile illness by using a Western immunoblot analysis and a newly developed ELISA.
Next, we determined the specific cell cycle regulators responsible for the cell cycle arrest induced by salinomycin by immunoblot analysis using antibodies specific to CDKs, cyclins, and CDK inhibitors.
