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To make this method applicable for the analyses of biological fluids of PhIP-exposed human subjects, it is now being improved by using immunoaffinity chromatography.
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We performed a preliminary extraction step [31], [32] followed by a purification step using immunoaffinity chromatography columns and reverse-phase high performance liquid chromatography prior to the testosterone radioimmunoassay.
Plasma-membrane components of this pancreatic cancer cell line were purified from plasma membrane fractions enriched by sucrose density-gradient centrifugation, using immunoaffinity chromatography on immobilized anti-human beta 2M antibody.
As an example Tu et al. [66] compared the protein content from plasma samples depleted using immunoaffinity chromatography with unfractionated samples.
We chose to further study two of these antibodies, 20D2 and 2D9, using immunoaffinity chromatography and mass spectrometry to identify their target proteins from Neospora lysates as above.
Antisera were purified using immunoaffinity chromatography.
We specifically isolated VLDL using immunoaffinity chromatography techniques.
Measurements of urinary concentration of 1-OHPG were performed using immunoaffinity chromatography and synchronous fluorescence spectroscopy [ 13].
Urine 1-OHPG concentrations were measured using immunoaffinity chromatography and synchronous fluorescence spectroscopy as previously described [ 16, 25].
To determine these patterns, we isolated and purified CEA proteins from human tumor tissues using immunoaffinity chromatography.
Urine 1-OHPG concentrations were measured using immunoaffinity chromatography and synchronous fluorescence spectroscopy and urine cotinine was measured using a dipstick test.
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