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The novel active fault tolerant control has been tested by using high fidelity simulators of aircraft and spacecraft systems, whilst the performances show the method robustness with respect to disturbance effects and measurement errors.
Due to the smooth terminal of target bands amplified by using high fidelity PCR enzyme KOD FX, it was necessary to add "A" tail to the terminal of the PCR products before TA cloning.
In order to identify the best temperature to ensure primer specificity, standard PCR on cDNA were performed with a gradient of annealing temperatures (ranging between 55°C and 65°C) for both target and reference primer pairs, by using high fidelity MyTaq DNA polymerase (BioLine).
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Future work could be focused on further refinement of the structural model reported here, for example by using higher fidelity atomistic force field models, or perhaps by using methods that facilitate the enhanced sampling of structural configurations.
However, these experiments are costly and time-consuming, and can be supplemented economically by using high-fidelity finite element models that can accurately mimic the response of the connections.
Thus, the probability of transformants having no untargeted mutations is considerably increased both by using high-fidelity Taq polymerases and by minimizing the size of the PCR fragment.
This cross-over study is the first to assess the difference in the actual force on oral structures during intubation attempts with the Pentax-AWS Airwayscope and the direct laryngoscope, by using high-fidelity simulators.
The gel-extracted DNA was then amplified for 17 cycles by PCR using high fidelity Phusion hot start polymerase (NEB, Ipswich, CA).
Specifically, all of the DNA fragments for the VP2 subunit proteins were amplified by PCR using high fidelity Platinum Taq polymerase (Invitrogen) with pVP2 as the target; the primers are summarized in Table 2.
Since this vector contains unique BglII, EcoRV and SpeI cloning sites to allow the gene of interest to be flanked by the 5' and 3' untranslated regions of the X. laevis β-globin gene, compatible BglII, EcoRV or SpeI sites were introduced for each aquaporin (depending on the restriction sites identified in the sequence) by PCR using high fidelity polymerase (Easy A, Stratagene).
The full-ORF VvDXS cDNA was amplified by PCR using high fidelity Phusion polymerase (Finnzymes) with the forward primer cVvDXS-fw 5-CACCATGGCTCTCTGTACG-3 and the reverse primer cVvDXS-rw 5-CTATGACATGATCTCCAGGGC-3, corresponding to the start and the end of the coding region of VvDXS.
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