Oil residues remained in the medium at various time points during growth was determined gravimetrically by using hexane extraction and weighted after evaporation (see "Materials and methods").
In view of the complex component of human urine, we develop a rapid and efficient pretreatment strategy for separation and purification of cocaine by using hexane extraction in real human urine samples within 3 min.
In order to overcome the high residual stress and low adherence of amorphous hydrogenated carbon (a-C H) films on steel substra-C H a thin amorphous silicon interlayer was deposited as an interfilmsbetween the substrate and a-C:H films pronuced by usteelhexane vapor asubstratessor gas.
The essence of seeds and fruits of the plant were extracted separately by using hexane as solvent as described in previous report [ 1].
The separated neutral lipid species, including TAG and FFA, were recovered from the plates using hexane, and quantified by GC/MS after conversion to their corresponding methyl esters by the methanolic-HCl method as described by Li et al. [ 46].
Serum levels of α-tocopherol, retinol, and five commonly reported carotenoids (α- and β-carotene, lutein, lycopene and cryptoxanthin) were extracted using hexane and analyzed by reverse phase HPLC, C18 column, and absorbance detector.
Both regular and defective onion-like carbon (OLC) nanoparticles were prepared by the arc-discharge process using hexane (C6H14) and ethanol (C2H5OH) as carbon sources.
Fractions were analyzed by TLC using hexane: ethyl acetate (70 30) as mobile phase and plates were stained with 2% anisaldehyde.
Following completion of the reaction (monitored by TLC), the solvent was evaporated under reduced pressure to obtain a crude product which was purified by PTLC using hexane:EtOAc (75:25) as eluent.
The reaction mixture is then extracted with ethyl acetate (4 × 25 mL), washed with water (2 × 50 mL), dried over anhydrous sodium sulfate, and the solvent evaporated under reduced pressure to give the red solid which was further purified by flash chromatography using hexane:EtOAc MeOH (10 9 1) as eluent.
