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To determine whether strains recovered from liver aspirate samples originated from gastrointestinal flora of patients, we investigated isolates from liver aspirate, nasal swab, saliva, and fecal samples by using genomic analysis.
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They then used genomic analysis to determine genetic kinship.
The taxadiene synthase gene (TXS) from transgenic A. annua plants, which were about 10 cm in height, was identified by PCR analysis using genomic DNA isolated by the CTAB method [ 21].
The absence of the intron in the NC2.1 strain was further confirmed by PCR analysis using genomic DNA as template, together with primers phac1 and phac2 (Table 5) using Taq polymerase (Fermentas).
Genotype analysis was performed by PCR using genomic DNA as the template.
The results of the aCGH were confirmed by quantitative PCR analysis using genomic DNA as the template with several sets of primers.
Mice with a deletion from Ube3a to Gabrb3 were identified by DNA genomic Southern analysis using genomic probes flanking and within the deletion interval as shown in the Figure 1E.
Genotyping was performed by polymerase chain reaction (PCR) analysis using genomic DNA isolated from toe snips of newborn mice.
To confirm if the collected cells were predominantly germ cells, we checked the DNA methylation level of the PEG10 DMR by COBRA (combined bisulphite restriction analysis) using genomic DNA extracted from the collected cells.
The sources of the cloned sequences were verified by highly stringent Southern blot analysis using genomic DNAs from both D. acuticephalum and the cephalopod Octopus ocellatus (data not shown).
By performing copy number alteration analysis using Genomic Identification of Significant Targets in Cancer (GISTIC) [ 30] in 16 types of cancers, we have found a diverse spectra of copy number alteration patterns, and show that only a few significantly altered genomic regions are present across multiple cancer types (Additional file 1: Figure S2).
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