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To characterize this type of RGC, we visualized SPIG1-expressing neurons by using genetically encoded green fluorescent protein (gfp) and examined their spatial organization, dendritic arborization, and axonal projection during the neonatal period.
These data provide information about cell growth and, by using genetically encoded fluorescent reporters, gene expression.
We considered that we could precisely monitor Ca2+ responses in vivo by using genetically encoded fluorescent Ca2+ monitoring probes which can be used for detecting rapid Ca2+ responses in living animals (Muto et al., 2013; Rose et al., 2014).
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Such variability can be accurately quantified by measuring levels of specific proteins in single live cells using genetically encoded fluorescent reporters and high throughput assays such as flow cytometry.
Using genetically encoded indicators including GCaMP5 and GCaMP6s delivered by AAV2/1 into the visual cortex, we demonstrate that high-quality two-photon imaging of large neuronal populations can be achieved and maintained in awake monkeys for months.
Tamsir, A., Tabor, J.J. & Voigt, C.A. Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'wires
How cells form global, self-organized structures using genetically encoded molecular rules remains elusive.
Joglekar, A. P., Salmon, E. D. & Bloom, K. S. Counting kinetochore protein numbers in budding yeast using genetically encoded fluorescent proteins.
Rabuka, D., Rush, J. S., deHart, G. W., Wu, P. & Bertozzi, C. R. Site-specific chemical protein conjugation using genetically encoded aldehyde tags.
Zhou, X., Li, S. & Zhang, J. Tracking the activity of mTORC1 in living cells using genetically encoded FRET-based biosensor TORCAR.
Recording of identified neuronal network activity using genetically encoded calcium indicators (GECIs) requires labeling that is cell type-specific and bright enough for the detection of functional signals.
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