Sentence examples for by using generic primers from inspiring English sources

Most of the amplicons corresponding to either HEV-A or HEV-B serotypes were generated by using generic primers 011, 055, 224, and 240 (18 ).

Reverse transcription nested PCR was conducted by using generic primers for flaviviruses, enteroviruses, and phleboviruses.

We conducted nested RT-PCR to detect a portion of the viral N gene by using generic primers for S segment as described (14).

A fecal sample was collected from the child and tested for norovirus by reverse transcription PCR (RT-PCR) by using generic primers that annealed to the polymerase region.

New STLV-3 (Cmo8699AB)- tax specific primers were designed to screen peripheral blood mononuclear cell DNA from all 63 hunters previously negative for sequences by using generic primers that can detect PTLV-1, PTLV-2, PTLV-3, and PTLV-4 (7 ).The assay could reliably detect 10 copies of STLV-3 (Cmo8699AB) tax plasmid sequences in a background of human DNA.

RNA was amplified by using generic primers for reverse transcription PCR (RT-PCR) (QIAGEN OneStep RT-PCR kits, QIAGEN) followed by heminested PCR (AmpliTaq Gold, Applied Biosystems, Foster City, CA, USA) with genotype-specific primers to identify G and P genotypes as described by Gouvea et al. (11 ) and Gunasena et al. (12 ), respectively.

Exponential amplification of circularized OCP (see Figure 2) was accomplished using generic primers P1 and P2, along with allele specific primer(s).

The approach we took to use generic primers for PCR is arguably the most viable method for determining the feeding hosts of tsetse flies.

Reverse transcription PCR was performed by using generic kobuvirus screening primers (UNIV-kobu-R/F) reported previously (4 ).

Consumers could save at least 50percentt of that amount by using generic versions, she said.

End stage heart failure can be appropriately managed by using generic palliative care skills [ 14].