Sentence examples for by using general primers from inspiring English sources

For molecular characterization, 16S rRNA gene was amplified through polymerase chain reaction (PCR) by using general primers (RS-1; 5′-AAACTCAAATGAATTGACGG-3′, RS-3; 5′-ACGGGCGGTGTGTAC-3′) (Rehmal et al. 2007).

PCR method for detection of animal derived materials in unknown feedstuff was developed by using general primer, relevant PCR system, and PCR condition.

The target comprising 16S and 23S rDNA was amplified and labeled by PCR (polymerase chain reaction) using general primers targeting a wide spectrum of bacteria.

The presence of HPV DNA in cervical cells was assessed using general primer-mediated GP5+/6+ PCR.

Screening for HPV was initially performed by using general PCR primers for HPV, which enabled detection of several HPV types (21 – 21 ).

Fragments of 16S rDNA genes were amplified by PCR using general bacterial primers, and bacterial populations were examined.

Each DNA sample was assayed for the presence of bacteria by PCR using general bacterial 16S rDNA primers described by [ 28].

The determination that these bacteria are Rickettsia is supported by: 1) Denaturating gradient gel electrophoresis (DGGE) analysis of the bacteria present in Er. eremicus using general 16S rRNA primers that target most known bacteria.

Most qPCR studies describing the gut microbiota typically do this by using a general bacteria primer and a few group-specific ones [ 4, 22].

Segments from these genes can be easily amplified by PCR using universal primers and sequenced.

Expression of CYP4 transcripts in digestive gland tissues was measured using general-cyp4V, general-cyp4BK, and general-cyp4BL primers.