Indeed, IL-10 deletion studies performed either by antibody inhibition or using gene knockout animals resulted in enhanced DTH response and antitumour activity (Nadler et al, 2003), while IL-10 knockout mice also showed prolonged survival and response in a syngeneic model of orthotopic bladder cancer (Riemensberger et al, 2002).
Previously, we examined loss-of-function phenotypes of yeast novel helicase-related genes, using gene knockout strains and characterized gene expression profiles by northern blotting.
Use of legitimate homologous recombination mechanisms in transfected B. bovis supports the future use of transfection methods for developing efficient gene function assignment experiments using gene knockout techniques.
Using gene knockout mice, it has been shown that ischemic PostC-induced decreased in infarct volume was triggered by activation of adenosine A1 and bradykinin B2 receptors [ 27].
Finally, we used gene knockout strains to validate the potential target genes related to ethanol tolerance.
However, because of the difficulty of transformation and gene targeting disruption in self-incompatible Brassica plants, the constructed model for self-incompatibility has not been confirmed by in planta experiments using gene-knockout self-incompatible mutant plants.
We validated the E.coli model, and MetaFlux in general, by using the gene knockout component of MetaFlux to simulate the effects of single-gene deletions in E.coli in glucose minimal medium.
Here, Halomonas TD01 was metabolically engineered using the gene knockout procedure based on markerless gene replacement stimulated by double-strand breaks in the chromosome.
Additionally, similar results were found when either gene was permanently inactivated using a gene knockout system.
The present study was designed to evaluate the contribution to learning of each of these genes by using single knockout (SKO) and double knockout (DKO) mice in an active avoidance test.
