Sentence examples for by using four steps from inspiring English sources

This improvement was achieved by using four steps of LMS pretreatment and NaOH washing [72].

In this article, we prepared the high index surface structures of Au nanocrystals supported on one-dimensional (1-D) MoO3 nanorods by using two steps ultrasonic probe irradiation method.

Human BM-MSCs were trans-differentiated into hepatocyte by using two steps protocol: differentiation step followed by maturation step.

Chitinase enzyme was extracelularly produced from Bacillus subtilis TV-125 and it was purified by using two steps.

Centrifugation of collected blood and harvesting serum samples were done by using three steps of centrifugation (800 g with 0.45 Am filtration, 1000 g, and 1500 g) to decrease lymphocyte to a minimum.

The treatment of contaminated soil was carried out using two steps: (1) injection by single air as marker, (2) washing with surfactant solution followed by washing with many water cycles.

Three repeat and 4R tau mRNA expression was detected by RT-PCR using One step RT-PCR kit (Qiagen,Crawley, UK) and primers: forward 5'-AAGTCGCCGTCTTCCGCCAAG-3'; reverse 5'-GTCCAGGGACCCAATCTTCGA-3'.

The system was first energy-minimized by using four consecutive rounds of 2000 steps of the steepest descent algorithm followed by 2000 steps of the conjugate gradient algorithm.

But he gives a lesson in handkerchief art by using four squares to illustrate the technical steps in reaching his final image.

Optimization studies are discussed by using two approaches: two-step optimization method with Sequential Quadratic Programming (TSOM case) and the multi-objective genetic algorithm.

Sequencing runs were done by using two different cleavage steps, which are two different four-nucleotide sequencing frames offset by two bases (two-step) or three bases (three-step) [ 29].