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The ROS level was assessed by using fluorescent probe DCFH-DA that detected H2O2.
In a recent investigation, the critical micelle concentration of the amphiphilic polymer was determined by using fluorescent probe.
It is possible that the elevated autophagy in irradiated MSCs may also serve as an adaptation to prevent the accumulation of ROS; hence, we detected the intracellular ROS level by using fluorescent probe dichlorofluorescin diacetate (DCF-DA).
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Fluorescence intensity was measured by using fluorescent probes: laurdan, DPH, and TMA-DPH.
Damage by these microbes was demonstrated by alterations of the activity of a constitutively expressed luciferase in the host, as well as by using fluorescent probes indicative of altered host cell permeability.
The generation of ROS by isothiocyanates was assessed by using fluorescent probes DCFH-DA by flow cytometry.
The instrument assays O2 and H+ levels by using fluorescent probes on a sensor cartridge that lowers to within 200 μm of the well bottom during a measurement cycle, thereby creating a trapped volume (7 μL) in which the measurement is made.
Chromosome instability in U251MG and D54MG cells was analysed by FISH using fluorescent probes for chromosomes 2 and 17 on days 2 (48 h) and 5 (120 h) after transfection with siRNA/control or survivin.
To confirm whether the centrosome amplification in this study indicated CIN or not, we analysed the centromeric copy number by FISH using fluorescent probes 2 and 17 on days 2 and 5. On day 2, the FISH analysis of D54MG cells revealed that >88% of cells showed three spots for chromosome 2 and 17 probes, thus indicating that these chromosomes are stable in D54MG cells.
Some of the experimental results for diffusion coefficients, determined by various methods using fluorescent probes, are summarized in Table 1.
Reactive oxygen species (ROS) production was measured by flow cytometry and microscopy using fluorescent probes for general ROS and mitochondrial superoxide.
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