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Pyrvinium is a quinoline-derived cyanine dye; the red fluorescence of pyrvinium can be observed by using fluorescent microscopy.
The viability of B. subtilis was assessed by using fluorescent microscopy by staining cells with Propidium iodide (PI) at 20× magnifications.
To measure transduction efficiency, the percentage of EGFP+ cells was determined by using fluorescent microscopy and flow cytometry 48 h after infection.
Fecal samples were concentrated by using the formyl ethyl acetate method, and sediment was examined by using fluorescent microscopy to detect oocysts (4 ).
Three sputum samples collected 3 days apart were digested and decontaminated with N-acetyl-L-cysteine, 4% NaOH; concentrated by high-speed centrifugation; stained with auramine-rhodamine; and evaluated by using fluorescent microscopy.
Other cell-based assay systems which use exogenous expression of genes (e.g. Escherichia coli β-D-galactosidase gene with and green fluorescent reporter) have previously been investigated for their potential in screening epigenetic drugs by using fluorescent microscopy and plate readers.
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The high cellular uptake (>80%) and localization of the copolymer vectors inside the cells were easily analyzed by tracking the fluorescence of polythiophene using fluorescent microscopy and cytometry.
Cellular EGFP expression was quantitatively examined by FACS analysis and visualized using fluorescent microscopy.
The size and morphology of the gut lumen was assessed at different stages by observing Nile Red incorporation using fluorescent microscopy (Leica).
We document DsRed regulation both by directly monitoring DsRed mRNA levels using q-PCR and by monitoring florescent levels of the protein using fluorescent microscopy.
Periplasmic localization was confirmed by detection of a fusion protein of Red Fluorescent Protein (RFP) and Caa using fluorescent microscopy.
More suggestions(15)
by using fluorescent polymerase
by using conventional microscopy
by using optical microscopy
by using advanced microscopy
by using fluorescent immunoassay
by using fluorescent lighting
by using fluorescent fusion
by using optic microscopy
by using fluorescent time-lapse
by using intravital microscopy
by using fluorescent immunoblotting
by using fluorescent microarray
by using fluorescent dextran
by using luminescent microscopy
by using confocal microscopy
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