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Gradients were confirmed by using fluorescent dextran in place of active protein (data not shown).
Injury with SLO also enhanced endocytosis in muscle fibers, detected by using fluorescent dextran as a fluid phase tracer.
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We analyzed the size-dependent effect of nanoparticles on permeation in this experimental model using fluorescent dextran molecules of different molecular weights.
Macropinocytosis was assayed using fluorescent dextran beads (70 kDa; 1 mg/ml) that were incubated with cells for 1 h, and subsequently washed out prior to fixation and staining.
Methods: Porcine retinal endothelial cells (PREC) were seeded into permeable inserts and cultured in 24-well plates that permit measurement of permeability using fluorescent dextrans.
Further, the impact of altered glycemia on BBB permeability was assessed in hCMEC/D3 – astrocyte co-cultures on Transwell supports using fluorescent dextrans (4 70 kDa).
Liposomes uptake was visualized by using fluorescent Dil marker [31].
Whitening pseudergates, identified by a whitening of the abdomen due to a gut purge before presoldier molting [21], and presoldiers within 24 hours after molting, were examined by retrograde staining using a fluorescent dextran.
Such limitations have been significantly improved by using dextran sodium sulfate (DSS) as an inflammatory agent.
Kobayashi et al. (2000) have used fluorescent probes for ROS detection [succinimidyl ester of dichlorodihydrofluorescein diacetate (H2DCFDA)] and endocytosis [TRITC-dextran] to monitor intracellular trafficking in phorbol myristate acetate-activated neutrophils.
PC346DCC selection medium was supplemented as described above, but depleted from androgens by using dextran-coated charcoal (DCC) treated FCS.
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