Sentence examples for by using fluorescence titration from inspiring English sources

All compounds were characterized and the selectivity, sensitivity, and stability of these dyes interacting with G-quadruplex were studied by using fluorescence titration, native PAGE experiments, FRET and CD analysis.

All compounds were characterized by using 1H, 13C NMR, IR, UV Vis and fluorescence spectroscopy, and DNA binding properties of these conjugates to calf thymus DNA were studied by using fluorescence titration, UV titration, isothermal titration calorimetry and CD analysis.

The complexes were also shown to reveal displacement of the ethidium bromide, a strong intercalator using fluorescence titrations.

A systematic effort to quantify changes in DNA binding affinity (dissociation constants) using fluorescence anisotropy titration led to the development of a p53 binding site predictor algorithm [ 60].

Using fluorescence anisotropy titrations, we measured the effect of every possible single base pair substitution of a consensus sequence on the affinity of the proteins for DNA.

We confirmed direct and reverse stoichiometries using fluorescence and anisotropy titrations and electrophoresis mobility shift assays.

(A ) Binding, measured using fluorescence anisotropy (points), during titration of rabbit muscle actin into 20 nM VCA-AF488.

Furthermore, we compare calcium titrations using fluorescence lifetime spectroscopy with the ratiometric approach and investigate potential non-FRET effects that may affect the fluorophores.

DNA titration was performed using fluorescence.

Fractions were analyzed for phosphate content using fluorescence of the phosphate sensor, MDCC-PBP, and the heparin content was determined by titration with Azure A (22).

An apparent Fe3+-siderophore/apo-FbpABp dissociation constant (Kd) corresponding to the equilibrium in eq 3 was determined by fluorescence titration using eq 4, where P represents FbpABp, Fe-sid represents both ferric native and ferric xeno-siderophores and P(Fe-sid) represents the FbpABp(Fe-siderophore) complex.