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Compounds A2 and A4 showed good hypoxic oxic fluorescence differential in vitro (V79 cells) by using fluorescence scan ascent.
Their evaluation for imaging tumor hypoxia was carried out in V79 cells, CHO cells, and 95D cells in vitro by using fluorescence scan ascent.
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Applied methods included electron paramagnetic resonance (EPR -, stopped-flow UV vis- and electronic circular dichroism (ECD -spectroscopy as wECD -spectroscopyexperiments by using fluorescence spectroscopy asd differential scanning calorimetry (DSC).
Monocyte-PEC cocultures with or without CD4+ cells were followed by analysis using fluorescence-activated cell scanning (FACS).
Fluorescent images were scanned using fluorescence microscope (Nikkon E800, Japan) and the images were captured by a digital camera.
Uptake of EC membrane by monocytes with or without scavenger receptor blockade was examined using fluorescence-activated cell scanning.
Animals analyzed by radiography were also examined by noninvasive, whole-body fluorescence imaging using a fluorescence scanning system (Nikon-SMZ1500; Nikon, Tokyo, Japan) with a fluorescence stereoscope attached to a Cool SNAP CCD camera (Photometrics, Tucson, AZ, USA).
All images were optimized by using a fluorescence microscope or a laser scanning confocal microscope (Nikon, http://www.nikonusa.com/).
Different fluorescence signals were imaged by using a laser scanning confocal microscope (Zeiss LSM 510 META).
A small piece of transgenic plant tissue was placed on the slide and immersed in a few drops of sterile Milli-Q water for visualizing the GFP fluorescence by using a confocal laser scanning microscope.
Clark, N. M. & Sozzani, R. Measuring protein movement, oligomerization state, and protein-protein interaction in Arabidopsis roots using scanning fluorescence correlation spectroscopy (scanning FCS).
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