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After 6 8 h, lattice-like vessel structures formed and then cells were stained with Calcein-AM (Invitrogen) and examined by using fluorescence microscope (Olympus).
After exposure of ZnO-NPs, fluorescence distribution images were observed by using fluorescence microscope equipped with a peltier cooled charge-coupled camera.
The mitochondrial ROS were visualized by using fluorescence microscope or analyzed by using FACS (Ex/Em: 510/580 nm).
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Analysis performed by dihydroethidium (DHE) revealed by direct estimation using fluorescence microscope the appearance of intense red fluorescence after 2 6 h of treatment with 25 μM parthenolide.
The changes occurred at the intracellular level during transfection especially post-endosomal step were monitored by fluorescence measurement using fluorescence microscope.
The fluorochrome H2-DCFDA, a general indicator of cellular ROS level, was used to ascertain ROS level by direct estimation using fluorescence microscope.
The ROS level was examined by detecting the fluorescence intensity using fluorescence microscope and microplate reader with excitation an emission wavelengths set at 500 and 529 nm, respectively.
At 36 hpi, viruses were harvested and further titered by end-point dilution assay using fluorescence microscope and X-gal staining for GFP or βγαλ ϖιρυσεσ ρespectively.
Morphological changes of ZR751 cells caused by PE extract were analyzed using fluorescence microscope after staining the cells with AO/EB.
High-intensity green fluorescence was observed in the transformants by using fluorescence and laser confocal microscope.
Detached INA-6 cells were examined afterwards by using a fluorescence microscope (Microscope Axiovert 25; Zeiss, Jena, Germany).
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CEO of Professional Science Editing for Scientists @ prosciediting.com