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In order to determine how cells adhere to a honeycomb-patterned film, focal adhesion of cardiac myocytes (CMYs) and endothelial cells (ECs) on the films were studied by using fluorescence labeling of vinculin.
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All polymorphisms were successfully detected by using fluorescence labeled Q-probe in a specifically amplified PCR product.
The trnasfection efficiency of siRNA in both Ishikawa and ECC-1 was examined by using fluorescence-labeling siRNA, si-GLO.
The terminator base can be identified using fluorescence labeling, isotope labeling, mass labeling for mass spectrometry and measuring enzymatic activity using an enzyme conjugate.
Figure 2 shows representative corresponding images of the tumour microvessels obtained by OPS imaging and by intravital fluorescence microscopy using fluorescence labelled dextran as a plasma marker to contrast the microvessels.
The uptake of TP5 by EBV-transformed B cells expressing HLA-DR molecules and the histogram of fluorescence intensities were observed by using fluorescent- labeled TP5, testifying the direct binding of TP5 to HLA-DR.
After hybridization detection used fluorescence labeling using tag-specific Cy3 and Cy5 dyes.
Fluorescently labeled cells were examined by using fluorescence microscopy.
By using fluorescence in situ hybridization (FISH) with rRNA-targeted, fluorescently labeled oligonucleotide probes, bacterial pathogens present in urine samples were identified within 3 4 h.
Dialyzed labelled protein was further analyzed by using fluorescence spectrophotometry to confirmed binding of fluorophore with protein bovine serum albumin.
The Flow-FISH method consists on labeling of telomeres with a fluorescent PNA probe followed by telomere fluorescence measurements on individual cells by using fluorescence-activated cell sorting (FACS) technology [ 26] (Table 1).
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