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High-intensity green fluorescence was observed in the transformants by using fluorescence and laser confocal microscope.
By using fluorescence and circular dichroism, we first determined qualitatively that HPrsc and HPr9 30 did bind to EIsc, the enzyme EI from S. coelicolor.
Cure reactions of the stoichiometric mixtures of diglycidyl ether of bisphenol A (DGEBA) and two very low molecular weight aliphatic polyether diamines (PED) were studied by using fluorescence and mid- and near-IR spectroscopic techniques.
Cells expressing Pdr5p-GFP fusion protein were examined by using fluorescence and differential interference contrast (DIC) microscopy (OLYMPUS BX51 microscope).
For instance, the folding pathway of an α-helical membrane protein in lipid vesicles has been kinetically studied by using fluorescence and absorption spectroscopy [19].
We have previously carried out chemical-denaturations of the His-tagged protein [25] by using fluorescence and CD at pH 5.9 25°CC), indicating that the protein is very stable.
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The silencing effect of M2-2 gene at the protein level was measured both qualitatively and quantitatively by using fluorescence microscopy and flow cytometry.
Cao et al. [ 59] investigated the interactions between polyamidoamine-C (a dendrimers) and curcumin by using fluorescence spectroscopy and molecular modeling methods.
Apoptotic and necrotic cells were detected by using fluorescence microscopy and Annexin-V-FLUOS staining kit (Roche, 11988549001).
The GdnHCl-induced and Urea-induced structural changes of apoNgb have been investigated by using fluorescence, ANS fluorescence, and far-UV CD spectroscopic techniques.
To explore the mechanism of TRIS-based separation of 1-propanol/2-propanol from their aqueous solutions, the interactions between different pairs of molecules were also investigated by using fluorescence analysis and Molecular Dynamic (MD) simulation.
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