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Validation of the 15K array by using field samples (n = 14) has shown 99% of parasite transcript detection from any of the samples.
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Further validations using field samples are underway.
Qualitative analysis of the samples was made by using field-emission scanning electron microscopy (FE-SEM Supra 25; Carl Zeiss, Jena, Germany).
The assay and the standard curve were evaluated by using spiked field samples.
The diagnostic accuracy of multiplex RT-PCR assay was evaluated by using 56 field clinical samples by multiplex RT-PCR, single RT-PCR and sequence analysis; and the specificity of multiplex PCR was verified by using constructed plasmids containing the specific viral target fragments of PRRSV and CSFV, respectively.
Parasitologic diagnosis of S. mansoni and soil-transmitted helminths (Trichuris trichiura, Ascaris lumbricoides, and hookworms) was performed in the field by using two stool samples collected on consecutive days and double Kato-Katz thick smears (2 × 41.7 mg of stools) for each mother and child.
For almost all tests, these parameters are first determined by using "spiked" samples and then evaluated under field conditions, often in comparison with other tests.
Ambient air samples were taken into special bags by using an odor sampling device designed for field sampling of odors.
The surface morphology of the sample was observed by using JSM-7600F fiemissionscanningnning electron microscope (SEM).
Chen et al. [ 19, 20] recently propose two improved Markov random field (MRF) algorithms, which can automatically assign weights to different data sources by using Gibbs sampling processes.
It also makes the model tractable by using negative sampling.
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