Sentence examples for by using existing primers from inspiring English sources

When insufficient underlying sequence information was obtained, the sample was entered into the secondary sequencing pipeline and reamplified by using existing primers or primers designed from the problematic sequence assembly itself.

Primers were designed using existing sequences for the two species as well as from house cat sequences downloaded from NCBI.

Marker saturation was carried out using existing and newly generated microsatellite markers (primer sequences available on request).

The genetic positions of forward and reverse primers were checked manually using existing genetic maps (http://wheat.pw.usda.gov).

Sequence-specific single-base variants are exposed by primer extension and, here, coupled to detection by MALDI-TOF mass spectrometry commonly used in clinical DNA diagnostics11, enabling the investigation of samples that were not suitable for analysis using existing methods.

pgiC and gapCp were initially amplified from all samples using PCR and existing primers [ 72, 73].

Use existing improvements.

Existing primers were used to characterize NAT2 genotypes at ten single-nucleotide polymorphisms (SNPs) in each buccal cell sample.

Most existing primer design software packages use sequence similarity searches to identify non-specific PCR products.

These or existing primer pairs may be tested for redundancy and specificity by electronic PCR against reference sequences using EMBOSS primersearch or individual primers mapped onto sequences using PATMAN.

Template (1 μl) was amplified by real-time PCR by using the primers listed in the Table 2 (Integrated DNA Technologies).