Sentence examples for by using established protocols from inspiring English sources

For PCR detection of the inserted gene segment, gDNA was extracted from tail or ear biopsies either by using established protocols [ 22] or commercially available kits (DNeasy® Tissues, Qiagen).

In situ hybridization was performed by using established protocols (8 ).

DNA was isolated from a pure culture by using established protocols (4).

The gene cluster replacement mutants and related complement strain were constructed by using established protocols (Coppi et al., 2001; Leang et al., 2003; Lloyd et al., 2003; Rollefson et al., 2009).

All analyses were conducted by the Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, using established protocols for the quantification of persistent environmental chemicals in serum.

In the late third trimester (135 days gestation; term 165 ± 10 days) AAV vector was administered under ultrasound guidance by the IP route using established protocols in telazol-sedated dams.

DNA prepared from whole blood samples was later analyzed by PCR for parasite species specific genotyping using established protocols [51] to confirm their infection status with the malaria parasites.

Serum concentrations of PBB 153, in parts per billion (ppb), were quantified by gas chromatography with electron capture detection using established protocols.

Two µg of amplified cDNA was used for library construction followed by pyro-sequencing on a Roche 454 GS-FLX (Roche, Basel, Switzerland) using established protocols [41].

DNA was extracted using established protocols.

Western blots were performed using established protocols.