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Well ordered FSM-16 mesoporous material was synthesized by using established procedures.
DNA constructions were carried out by using established procedures [31] or following the instructions provided with specific reagents.
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TruD overexpressed in E. coli BL21 (DE3) cells and purified to homogeneity (as judged by SDS-PAGE) using established procedures[ 8] was used for all analyses.
Docetaxel loading in the PLGA PEG NPs were measured by HPLC (Agilent Technologies, 1100 Series) using established procedures.
Equivalent quantities of protein (20 μg) were separated by SDS-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore) using established procedures.
Kinetic parameters evaluated using this technique compare favourably with those determined using established procedures.
A platinum (Pt) protective layer was deposited over a region of interest (ROI) using established procedures.
The network reconstruction was converted to a constraint-based model using established procedures [ 13].
The probe was hybridised to S. mansoni chromosomes using established procedures [ 78].
We are reducing this burden by (1) asking practices to use established procedures and software to identify eligible patients for vaccination.
We have used established procedures to test urinary concentrating and diluting capacity [ 21].
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