Assays were performed in triplicate to measure NF-kB by using equal amounts of protein from the cell extracts.
OC and the internal standard, OCD3, were separated by using: equal amounts of the mobile phases H20 and ACN, both containing 0.1% (v/v) formic acid; a flow rate of 0.200 ml min−1 and a Waters Acquity™ UPLC BEH C18 (2.1×50 mm, 1.7 µm particle size) analytical column.
Two pools (normal pool and mutant pool) were constructed by using equal amount of DNA of WT and F2 mutant with 10 individuals for each.
Real-time PCR amplification was carried out in a final volume of 15 μl by reaction using equal amounts of cDNAs (2 μl - 200 ng/μl) as template, 0,2 μM of each primer and 7,5 μl of Maxima SYBR Green/ROX qPCR master Mix (fermentas, USA) at the following conditions: 50°C for 2 min, 95°C for 10 min, 45 cycles of 95°C for 2 min, 62°C for 30 seg, 72°C for 30 seg.
FPU were measured by the robot using equal amounts of filter paper (Whatman No. 1) different amounts of a typical mixture of commercial enzymes devised for biomass digestion (Celluclast and Novozyme 188, Novozymes A/S, Bagsvaerd, Denmark) The hydrolysis was carried out for 1 hour at 50°C, and the FPU calculated based in the amount of glucose released as detailed previously [ 19].
Changes in BrdU incorporation were assessed by slot blot analysis using equal amounts of genomic DNA isolated from DMSO-, 898- or 233-treated cells.
Tissue samples obtained from left ischemic hemispheres at 24 h after stroke (n=4 animals per condition) were homogenized in 50 mmol/l Tris, pH 8.0, 150 mmol/l NaCl, 1% Triton X-100 and protease inhibitors, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using equal amounts of protein.
It projects that by 2040, global economic growth will be fueled using equal amounts of coal, oil, natural gas, and renewables.
Using equal amounts of each microorganism in donor whole blood.
Make duxelles using equal amounts of diced mushrooms and onions.
The optimum induction time for all the UGTs was monitored by western blot using equal amount of proteins and antibodies raised against the anti-ExpressTM epitope present between the 6x Histidine tag and the multiple cloning site of the construct.
