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We performed this study to determine whether subtype H5N1 virus circulation in Cambodia could be better monitored by using environmental sampling in LPMs.
However, one study defined the distance depending on residents' odor complaints [ 27], while others defined it by using environmental sampling [ 34, 35].
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However, the current study is an in situ evaluation using environmental sampling.
Although we did not carry out additional experimental validation of our novel and non-degenerate universal primer set by using other environmental samples rich in eukaryotic and mitochondrial rRNA genes, the results in this study indicate that our primer set is highly expected to be applicable to the analysis of many diverse microbial communities.
Environmental samples (infant cup, infant toys and spoon, and kitchen floor area) and hand swabs (mother's and sibling's dominant hand) were taken by using commercial environmental sponge sampling kits (Bacti Sponge Kit; Hardy Diagnostics, Santa Maria, CA).
The best (most efficient, valid and reliable) sampling schemes use environmental knowledge to guide the sampling.
Sterile electrostatic cloths were used for environmental sampling and information regarding the surface sampled was recorded.
Subsequently, analysis of samples was continued by following the same approach used for environmental samples.
A total of 25 environmental PM2.5 measurements were analyzed at 5 different locations by using a dichotomous sampler, and a total of 55 personal PM2.5 measurements were analyzed from inspectors by using PM2.5 personal sampler.
The 15-min TWA Leq was collected by industrial hygienists at 337 locations around the company using short-term environmental sampling.
This study suggests that an infection control programme based on active surveillance and strict adherence to hand disinfection and gloves use policies and supported by environmental sampling and molecular analysis is effective in controlling NICU multi-clone P. aeruginosa outbreaks.
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