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The goal of this study was to investigate M. tuberculosis in their in vivo environment by using differential staining techniques that target specific components of the M. tuberculosis bacillus, and whether certain bacillary populations could be identified in the different microenvironments tested in vitro and in vivo.
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Cell morphology was observed by using differential interference contrast (DIC).
Mitochondrial-enriched RNA samples were prepared by using differential centrifugation.
The inflammatory response associated with MWCNT exposure was characterized by neutrophilia as determined by BALF cell differentials and by using immunohistochemical staining with an anti-neutrophil antibody, which revealed neutrophils near terminal bronchioles and in proximity to macrophages containing O-MWCNTs.
As previously described, FPG Hoechst- Giemsa technique modified by De Salvia et al. [ 15] was used for differential staining of sister chromatids.
Expression of the SP receptor (NK1) was detected by using immunocytochemical staining and PCR.
The cells were also cytospun onto glass slides for differential counting by using Diff Quick stain (Dade Behring, Newark, DE, USA).
This is exemplified by differential staining using different antibodies to MUC1 and to endothelin.
The purity of isolated neutrophils was determined by differential staining using a commercial May-Grunwald Giemsa stain (Diff-Quick, Baxter Healthcare, UK) and light microscopy and was routinely greater than 97%%.
To perform cell differentials, cells were fixed on glass slides by using cytospin and stained with geimsa.
Leukocytes also infiltrated the treated mucosa and the number and composition of leukocytes obtained by cervicovaginal lavage (CVL) were determined by differential staining and flow cytometry.
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