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Hence, in a recent study we showed that data variation introduced by biological replicates of the mouse retina is small compared to the variation caused by using different technologies [ 4].
In the past decade, there have been considerable improvements in the way that human breast tumours are identified and characterized, uncovering biomolecular alterations by using different technologies (like DNA assessment and mutation screening, gene-expression and microRNA, proteomic-metabolomic-degradomic profiling, etc).
To guarantee the GMO traceability, a key factor in the implementation of these regulations, several strategies, categorized as indirect (protein-based methods) or direct (DNA-based methods), have been developed to detect GMO in food/feed samples by using different technologies.
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Voice and data networks have traditionally been served by different networks using different technologies.
The GEP partnership in many ways follows the successful pattern established by GCAT, albeit using different technologies.
Analyte-specific interferences induced by a specific therapeutic (with a defined molecular nature) can be identified easily and reproducibly by spiking of the drug in relevant concentrations in the sample of interest, followed by its quantification using different technologies and/or antibody pair combinations.
We acknowledge that the sample size is a limiting factor of this study, and while similar results were reported by independent groups using different technologies [ 7, 9, 33, 45- 47], larger studies will be needed to precisely determine the genomic effects of EBV transformation.
These efforts were also preceded or accompanied by transcriptome sequencing efforts using different technologies [61 64], in support of gene prediction [65], but also for depicting transcriptional processes and define cell functionality in physiological, pathological, or stress conditions.
However, their use all together into the same detection system is hampered by several drawbacks: they are using different technologies (e.g. TaqMan® and SYBR®Green), they are using different protocols and they were developed for different purposes and validated following different ways.
Meta-analysis has already been extensively used in genome-wide association studies to identify the susceptibility loci for complex disorders and cancer gene expression signatures when comparing multiple studies carried out by different groups or using different technology platforms.
However, our work further validates the use of oncotype markers by confirming their prognostic value by studying them at the protein level using different technology.
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